Mitochondrial function in neuronal and glial cells is disrupted after cardiac arrest. It is commonly accepted that mitochondrial health indicates the overall health of a cell. However, it is not currently known if different cell types in the brain (neurons and glial cells) respond to injury differently. This project uses a four-pronged approach to explore the cell-specific responses to pediatric cardiac arrest to inform future studies targeting specific cells in post-cardiac arrest treatment. Mitochondrial dynamics, morphology, and overall content in response to injury will be assessed in pre-clinical tissue. Furthermore, the techniques developed in this study lay the foundation for assessing cellular health and its relationship to mitochondrial health in future studies.