Subglottic Stenosis is a narrowing of the airway common in pediatric patients who have undergone prolonged intubation. The current standard of care; laryngotracheal reconstruction (LTR) using rib cartilage, has many drawbacks and thus it has necessitated the creation of a biomaterials solution to repair the airway. This project follows the in vitro characterisation of a decellularized meniscus scaffold reinvaded with cartilage progenitor cells for LTR and its subsequent in vivo implantation in four-month-old Yukatan Miniature Pigs. The results demonstrate that decellularized meniscus scaffolds (MEND) provide a promising substitute for rib cartilage for the future of LTR procedures.