Anesthetics such as propofol, etomidate, and ketamine are responsible for producing unconsciousness in patients. Mitochondria within neurons is responsible for energy dynamics, and its location within the neuron is critical. The effect of these anesthetics on mitochondria delocalization in neurons has not been properly investigated. Furthermore, transport proteins such as kinesin transport mitochondria to different parts of the cell. Previous studies have shown that kinesin motor proteins can be affected by anesthetics, altering the placement of mitochondria in the cell. By using a four fluorophore immunofluorescence stain, we can visualize different parts of the zebrafish brain. The use of a zebrafish model provides a model for exploring these critical research questions.