This summer, my research focused on using CRISPR/Cas9 technology to potentially delete the myostatin gene in mouse and human cell lines, aiming to induce muscle hypertrophy. Myostatin, also known as Growth Differentiation Factor 8 (GDF-8), is a crucial negative regulator of muscle growth. My research included designing sgRNA primers to cut at specific sites to delete the myostatin gene. The deletion was tested through DNA extraction, PCR, and gel electrophoresis. This project is important for advancing treatments for muscle-wasting conditions and improving muscle recovery in athletes. Through this work, I explored the complex process of gene editing and its significant implications in medicine and athletes.