PERK is one of the four kinases responsible for activating the Integrated Stress Response (ISR) pathway, which restores cellular homeostasis via inhibition of cap-dependent protein translation. The Ensembl database has identified several transcription isoforms for the PERK gene, EIF2AK3. Preliminary results support that some of the identified transcripts are predicted to produce PERK isoforms. Thus, as the first step of a bigger project, we aim to rule out whether the additional bands observed via a western blot for PERK are due to phosphorylation or non-specific bands. Ultimately, further research to better distinguish the western blot (WB) bands and the use of mass spectrometry studies is required.