Altering erythrocyte membrane components can be clinically relevant. Red blood cell cytoskeletons contain many different components, and altering them can result in many mutations. This project focuses on Tropomyosin 1 (TPM1), a protein stabilizing actin in red blood cells and other cells in the body. Mutations in almost all other genes in the red blood cell have been studied, but not for TPM1. Through a series of breedings, a Gene Trap Reporter mouse model was created, as well as a conditional knockout mouse model to remove TPM1 in red blood cells in order to study its effects in blood cells. A total knockout of TPM1 is embryonically lethal due to cardiac defects. With each mouse model, tropomyosin levels and blood phenotypes were analyzed, displaying a general correlation between decreased levels of tropomyosin 1 and increased levels of elliptocytosis and abnormal red blood cell phenotypes.