Briefly, our lab investigated MHC class II-restricted HIV-1 antigen presentation between two human cell types: CD4+ T cells and dendritic cells. In further detail, we wanted to gauge and analyze the antigen processing and presentation capabilities of these cell types. In order to do so, we infected these cells with various forms of HIV-1, such as ones that cannot replicate or fuse to target cells (as required for infection), or prepared them with a specific HIV-1 peptide. We then plated these infected cells with "responding" CD4+ T cells—which were used to measure whether the infected cells were presenting HIV-1 antigen—and conducted flow staining to analyze the outcomes.