This summer, I worked with William Han in the Lee Visualization Lab. Our project was the first step towards the overall goal of developing a fluorescent imaging dye that will improve surgical resections indiscriminately for both low-grade and high-grade brain tumors. Based on previous studies, we wanted to characterize the profiles of certain amino acids transporters at the blood brain tumor barrier to determine whether they are overexpressed at the vasculature and if they could serve as a potential target for our fluorescent agent. William and I performed immunohistochemistry experiments on both low grade and high grade glioma tissues to firstly determine the optimal dilution for staining and secondly confirm overexpression. We then imaged all the slides under a microscope at 10x and 20x magnification.