Obesity is commonly described as adipose tissue expansion due to a build-up of lipids from excess nutritional intake. Adipose tissue expansion can impede the functions of vital organs causing various types of metabolic disorders such as type 2 diabetes and cardiovascular conditions like coronary heart disease. However, adipose tissue expansion not only occurs in obesity but also in contexts of skeletal muscle injury. During muscle regeneration after injury, the formation of intramuscular adipose tissue (IMAT) occurs within the fibers of skeletal muscles. In obesity, lipid accumulation in IMAT can lead to decreased muscle strength and inflammation. Our project aims to understand how FAPs react to injury and obesity and how this may affect muscle health through in vitro experiments of FAPs.