Due to the burdens of genetically engineering stem cells, inefficient and incomplete CRISPR gene perturbations in neurons have plagued the reliability of genetic screens in complex cell types such as neurons. The objective of this thesis was to establish a platform to generate iPSC cell lines with stable expression of constitutive and inducible CRISPR machinery for genetic screens with minimal genetic engineering. CRISPR integrated KOLF2.1J cells show stable dCas9 expression in long term culture and post freeze/ thaw following PiggyBac transfection. KOLF2.1J cells show a similar decrease in dCas9 expression in differentiated neurons to the current most commonly used cell line for gene perturbations in neurons, the WTC11 line. Immunofluorescence and western blot data show that dCas9 is expressed in the nucleus in iPSCs and expression is excluded from nuclei in differentiated neurons, and that cleavage of the dCas9 protein is occurring in neurons. This suggests that inability to localize at the nucleus and cleavage of the dCas9 protein are two potential explanations for loss of dCas9 expression and reduced gene silencing in neurons. Engineering new cell lines that can either improve nuclear localization or prevent cleavage of dCas9 in neurons would be the next step in establishing this platform of generating stable constitutive and inducible CRISPR machinery for genetic screens.