Disruptions of circadian rhythm in glucose metabolism can lead to Type 2 Diabetes. In this experiment, we studied the effects of knocking out Dec1, a transcriptional repressor found in mature β-cells that inactivates Core Clock genes.

Dec1 was knocked out at 8-10 weeks, using Tamoxifen, in Mouse Insulin Promoter (MIP)-CreERT Dec1 fl Mouse β-cells, which allows us to study the effect of Dec1 in insulin secretion and blood glucose responsiveness of adult mice.