Conventional 3D quantification of the aortic valve apparatus in pre-operative transesophageal echocardiography (TEE) involves interaction with a volume-rendered image and cross-sectional planes on a standard computer display. However, the use of virtual reality (VR) could improve visualization, depth perception, and ease of measurement in 3D images through immersive interaction, augmenting surgical planning for structural heart disease. The objective of the study is to evaluate quantitation of aortic root dimensions in pre-operative TEE using VR. Future work may integrate VR into the clinical workflow for diagnostics and surgical planning.