Fall Research Expo 2020

The Effects of General Anesthetics on Synaptic and Cognitive Function

This past summer, I had the opportunity to work under the mentorship of Dr. Huafeng Wei and Dr. Maryellen Eckenhoff as part of the Department of Anesthesiology at the Perelman School of Medicine. Previously, the lab conducted research into the effect of anesthetics on synaptic and cognitive function via the inositol 1,4,5-trisphosphate receptor (IP3R), a calcium channel in the endoplasmic reticulum. However, the original investigator moved on and the results went unpublished. Our goal for the summer involved preparing a manuscript for publication communicating these results.

 

As I am new to the study of biological pathways, one of the greater challenges I faced involved learning and understanding the background material and experimental methods. Thus, one of my first tasks involved reading relevant literature and gathering potential references for the paper. Once I built a solid understanding of the experiment, I began to sort through the original raw data in external hard drives, using the lab notebooks to ensure that each set of data was accounted for in the experiment. With the help of my mentors, I reanalyzed the raw data in a statistics software, and based on the statistical significance of a result, I compared the conclusions to the initial hypothesis. My responsibilities also included creating figures to illustrate the experimental timeline and the pathways we investigated. Using the papers I found for reference, I practiced writing the abstract for the manuscript.

 

Working remotely, I honed critical organization and communication skills by keeping regular correspondence with my mentors and the original investigator, providing progress updates at weekly lab meetings, and creating presentations on the results of my data mining. In addition, I have had the opportunity to work with software such as AI, EndNote, and GraphPad Prism.

 

Through this program, I gained valuable insight on the process of constructing a scientific manuscript. I hope to continue my work with the lab during the academic year.

PRESENTED BY
PURM - Penn Undergraduate Research Mentoring Program
Engineering & Applied Sciences 2023
Advised By
Huafeng Wei
M.D., Ph.D.
Maryellen F. Eckenhoff
Ph.D.
Join Katherine for a virtual discussion
PRESENTED BY
PURM - Penn Undergraduate Research Mentoring Program
Engineering & Applied Sciences 2023
Advised By
Huafeng Wei
M.D., Ph.D.
Maryellen F. Eckenhoff
Ph.D.

Comments

What was the ultimate relation between anesthetics and the IP3R functioning? Do you know what these findings will lead to in the future? 

Such a cool project! How did you get interested in this particular topic and do you plan on continuing to work with IP3R in the future (when labs reopen for undergrads)? 

Very interesting to see a project focusing on the completion of an already completed experiment. How long do you think until you would have finished the whole manuscript? Also, what prompted you to pursue this research instead of a project working on something new/ completing experiments? 

Very interesting to see a project focusing on the completion of an already completed experiment. How long do you think until you would have finished the whole manuscript? Also, what prompted you to pursue this research instead of a project working on something new/ completing experiments?