Fall Research Expo 2020

Recellularizing Engineered Cartilage for Pediatric Airways

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Decellularization shows promise as a scaffold fabrication technique for tissue engineering, but cartilage poses recellularization challenges with its dense matrix and sparse cell population. We evaluated cell reinvasion using image analysis with ImageJ in a new scaffold derived from cartilage decellularized using a novel technique. For different seeding protocols we compared cell density, invasion distance, and nuclei to nuclei separation. Scaffolds seeded with 200k cells and incubated for 1 week in a Transwell plate and 1 week in a Bioreactor proved to be the best condition. Next, we will assess differentiation in these scaffolds.

PRESENTED BY
Leah Levin
PURM - Penn Undergraduate Research Mentoring Program
College of Arts & Sciences 2023
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Advised By
Riccardo Gottardi
gottardir@email.chop.edu
https://www.gottardilab.com/
Paul Gehret
gehretp@seas.upenn.edu
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PRESENTED BY
Leah Levin
PURM - Penn Undergraduate Research Mentoring Program
College of Arts & Sciences 2023
View Profile
Advised By
Riccardo Gottardi
gottardir@email.chop.edu
https://www.gottardilab.com/
Paul Gehret
gehretp@seas.upenn.edu

Comments

Fascinating and important work, Leah.  Is there a reason why you are using stem cells as models for cell migration in this novel system?  When (hopefully) this moves further toward implementation in animal models, etc., is there a comparable pool of tracheal stem cells that can be transplanted or will they be more mature/differentiated? 

Hi Leah, I found your project very interesting and innovative. I think tissue engineering is a really cool technique that, once streamlined, could help many people. 

Thanks, Elisa

I found your poster to be extremely effective, and your video was so engaging! Even as a non-STEM major, I'm so interested in hearing more about your findings! Great work